.png)
.png){kind=icon}
IDENTIFIKASI BAKTERI PADA SERASAH DAUN MANGROVE YANG TERDEKOMPOSISI DI BANDAR BAKAU KOTA DUMAI
Abstract
Bacteria has an important role in decomposition and productivity activities in mangrove ecosystem. The study about identification of decomposing mangrove litter bacteria in Dumai has never been done. The aim of this study is to determine Total Plate Count and identify the dominant bacterial species from decomposing Rizhopora sp, Xylocarpus sp, dan Avicennia sp litter in Dumai. The method used in this research was survey method, bacterial isolation was done by pour plate method which is used in total calculation of bacteria was Total Plate Count (TPC), and identify the dominant bacterial in mangrove litter. The total bacteria which gained on Avicennia sp leaf litter are 0,64 x 105 Cfu/ml. On the other hand, the total bacteria from Rhizopora sp leaf litter has a lower number of colonies that is 0,55 x 105± 0,01 Cfu/ml. The bacterial identification test was carried out by gram staining method and morphological observation by microscopy. The dominant bacterial species in three mangrove species (Rizhopora sp, Xylocarpus sp, dan Avicennia sp) are Micrococcus sp dan Aerococcus sp.
Keywords : Total Plate Count, Micrococcus sp, Aerococcus sp, Organic Matterial
Full Text:
PDFReferences
Brown, MS. 1984. Mangrove litter production and dynamics. 7n:Snedaker, C.S. J.S. Snedaker eds. The mangrove ecosystem Research methods monograph on oceanographic methodology. UNESCO. 231-238.
[BSN] Badan Stadardisasi Nasional.2006. SNI 01- 2332.3-2006. Tentang Penentuan Angka Lempeng Total (ALT) pada Produk Perikanan. Jakarta
Cowan, ST. 1974. Cowan and Steel’s Manual for the Identification of Medical Bacteria. 2nd Edition, Cambridge University Press, Cambridge. 67-83.
Das, S, Lyla, PS. and Khan S. 2006. Marine microbial diversity and ecology: importance and future perspective. Current Science. 90(10) : 1325- 1335
De Vuyst, L, dan Vandamme, E. J. 1994. Antimicrobial potential of lactic acid bacteria. In Bacteriocins of lactic acid bacteria (pp. 91-142). Springer US.
Gunathunga, SU, dan Rathnayake, IVN. 2017. Endophytic Bacterial Diversity of Mangrove Leaves. International Research Symposium on Pure and Applied Science. Faculty of Science, University of Kelaniya. BO-27
Hanafiah, KA. 2007. Dasar-Dasar Ilmu Tanah. PT Raja Grafindo Persada. Jakarta. hal. 7; 167; 211
Harpeni E. 2007. Eksplorasi Bakteri yang Berasosiasi dengan Karang Lunak sebagai Alternatif Sumber Senyawa Bioaktif. Uji Hayati Antibakteri. Biosfera. Vol 24 No 3.
Harrow, GI. and Feltham RKA. 2003. Cowan and Steel’s Manual for The Identification of Medical Bacteria. 3th ed. Cambridge University Press, United Kingdom, 331 p.
Holt, JG, Krieg NR, Sneath PHA, Staley JT dan Williams ST. 1994. Gram negative aerobic microaerophili rods and cocci. Group 4, In: "Bergey's Manual of Determinative Bacteriology, 9th ed.", pp: 93-153. Williams & Wilkins, Baltimore, USA. 217-549 p.
Kapludin, Y. 2012. Karakteristik dan Keragaman Biota pada Vegetasi Mangrove Dusun Wael Kabupaten Seram Bagian Barat. Universitas Darussalam, Ambon.
Khusnuryani, A., Martani, E., Wibawa, T., Widada, J. 2015. Karakterisasi Bakteri Pendegradasi Fenol Dan Pembentuk Biofilm Dari Sumber Alami Dan Artifisial. Kaunia Vol. XI No. 1: 40-50
Nofu, K., Khotimah,S., Lovadi,I. 2014. Isolasi dan Karakteristik Bakteri Pendegradasi Selulosa pada Ampas Tebu Kuning (Bagasse). Jurnal Protobiont Vol 3 (1): 25 – 33.
Paul, J dan Varma AK.1993.Hydrolytic Enzyme Production in Micrococcus roseus Growing on Different Cellulosic Subtrates. Applied Microbiology 1993, vol. 16, hal. 167-169.
Reanida PP. 2012. Eksplorasi Bakteri Selulolitik dari Tanah Mangrove Wonorejo, Surabaya [Skripsi]. Surabaya : Universitas Airlangga
Ritonga, DW. 2012. Keanekargaman Bakteri Serasah Daun Rhizophora apiculata yang Mengalami Dekomposisi Pada Berbagai Tingkat Salinitas di Kota Pari Pantai Cermin Sumatera Utara.[Tesis]. Universitas Sumatera Utara. Medan
Saseeswari A, Kanimozhi G, Panneerselvam A. 2016. Bacterial Diversity of Soil in Karankadu from East Coast of Tamil Nadu, India. International Journal of Current Microbiology and Applied Sciences. Vol. 5 No 4. 750-756
Singh A, Hayashi K. 1995. Microbial cellulase, protein architecture, molecular properties and biosynthesis. Advance Applied Microbiology. 40, 1-44.
Suryani, Yani. 2011. Bioremediasi limbah merkuri dengan menggunakan mikroba pada lingkungan yang tercemar. ISSN 1979-8911. Vol V No 1-2
Tyas DE, Widyorini N, Solichin A. 2018. Perbedaan Jumlah Bakteri dalam Sedimen pada Kawasan Bermangrove dan Tidak Bermangrove di Perairan Desa Bedono, Demak. Jurnal Maquares. 7(2):189-196
Wanhlem, Noraphat, Chobert, Jean-Marc H-kittikun dan Aran. 2014. Bacteriocin-producing lactic acid bacteria isolated from mangrove forests in southern Thailand as potential bio-control agents in food: Isolation, screening and optimization. Food Control. 41. 202–211.
Yunita, M., Hendrawan Y, dan Yulianingsih R. 2015. Analisis Kualitatif Mikrobiologi Pada Makanan Penerbangan (Aerofood ACS) Garuda Indonesia Berdasarkan TPC (Total Plate Count) Dengan Metode Pour Plate. Jurnal Keteknikan Pertanian Tropis dan Biosistem. 3(3): 237-248.
Yuspita, NLE, Putra IDNN, Suteja Y. 2018. Bahan Organik Total dan Kelimpahan Bakteri di Perairan Teluk Benoa, Bali. Jurnal of Marine and Aquatic Sciences. 4(1): 129-140.
DOI: https://doi.org/10.35308/jpt.v7i2.2349
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Putri Wening Ratrinia, Nirmala Efri Hasibuan, Aulia Azka, Sumartini Sumartini, Apri Mujiyanti, Kurnia Sada Harahap, Muh Suryono
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Jurnal Perikanan Tropis (print ISSN 2355-5564 ;online ISSN 2355-5572), is published by the Faculty of Fisheries and Marine Science Universitas Teuku Umar, Indonesia .
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License