Effective Use of Mimosa pudica Leaf Extract on Cyprinus carpio Infected with Aeromonas hydrophila
Abstract
This study aims to determine the effect of giving Mimosa pudica leaf-soaking extract to Cyprinus carpio fish infected with Aeromonas hydrophila. This research was conducted in the Laboratory of Fish Hatchery and Breeding, Faculty of Marine and Fisheries, Syiah Kuala University and the method used a complete randomized design (CRD) with 4 treatments (A: Mimosa pudica extract 0%, B: Mimosa pudica extract 3%, C: Mimosa pudica extract 5%, D: Mimosa pudica extract 7%). The results of this study indicate that Mimosa pudica extract affects the clinical symptoms of Cyprinus carpio. Data from ANOVA analysis showed that the administration of Mimosa pudica extract had a significant effect (P<0.05) on survival with each treatment of 36.67±11.54% in treatment A, in treatment B of 56.67±5.77%, in treatment C of 66.67±5.77% and in treatment D of 83.33±5.77%. Leukocyte results in all treatments showed differences in values where each was in treatment A at 29.5 104 cells/mm3 , in treatment B at 19.5 104 cells/mm3, in treatment C at 15.4 104 cells/mm3, and in treatment D at 7.21 104 cells/mm3. The haemoglobin results of all treatments also had different values, namely in treatment A of 3.4 g/dl, treatment B of 4.2 g/dl, treatment C of 5.2 g/dl, and treatment D of 6 g/dl. The conclusion from the results of this study is that 7% Mimosa pudica leaf extract shows the best results on clinical symptoms, survival, leukocyte count and haemoglobin levels.
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Anderson, D. P and A. K. Siwicki. 1993. Basic Hematology and Serology for Fish Health Program. In: Symposium on Disease in Asia Aquaculture Aquatic Animal Health and Environment, Thailand, pp. 11-193.
Anggita, A., Fakhrurrazi, & A. K Harris. 2018. Anti-bacterial test on ethanol extract of putri malu (mimosa pudica) leaves against pseudomonas aeruginosa bacteria. Jumvet. 2(3):411-418.
Anugrah MH. 2018. Adlibitum feeding of different foods on the growth and survival of tilapia (Oreochromis niloticus) fry. Faculty of Agriculture. University of Northern Sumatra.
Ardika, P. U., E. Nofyan, E. Junaidi. 2016. Effect of Coal Mine Acidic Water Waste on the Number of Erythrocytes, Hemoglobin and Leukocytes of Goldfish (Cyprinus carpio L.). Proceedings of SEMIRATA MIPA 2016; BKS-PTN Barat, Palembang May 22-24, 2016. Page 2413-2417.
Buda, S. N., Y. Koniyo, A. Lamadi. 2023. Effectiveness of Belimbing Wuluh Fruit Extract (Averhoa blimbi L) in Inhibiting the Growth of Aeromonas hydrophila Bacteria in Goldfish (Cyprinus Carpio). Multidisciplinary Scientific Journal, 2 (2); 164-170.
Dermawan, Aliza, Anggraini, R. 2016. Identification of Aeromonas hydrophila bacteria using microbiological tests on Dumbo catfish (Clarias gariepinus) cultivated in Baitussalam District, Aceh Besar Regency.
Unsyiah Maritime and Aquatic Student Scientific Journal 1 (2): 270-286.
Dianti, L., S.B. Prayitno, & R.W. Ariyati. 2013. Non-specific resistance of carp (Cyprinus carpio) soaked in jeruju leaf extract against aeromonas hydrophila bacterial infection. Journal of Aquaculture Management and Technology. Vol 2 No. 4 pp 63-71.
Fadlian, B. Hamzah, and P. H. Abra. 2016. Test the effectiveness of putri malu plant extract (Mimosa pudica Linn) as a natural preservative for tomatoes. Journal of Chemistry Akademika. 5(4): 153-158.
Gilani, J. Tolouei, A. Goudarztalejerdi, M. Yavari, M. N. Kalourazi. 2021. Isolation and Identification of Aeromonas hydrophila from Cyprinidae Suspected with Hemorrhagic Septicemia in Pools of Warm Water Fishes in Gilan Province. Int J Nutr Sci 2021;6(1):52-58.
Hamidah, M. N., Rianingsih, L and Romadhon. 2019. Antibacterial activity of isolates of lactic acid bacteria from peda with different types of fish against E. Coli and S. Aureus. Journal of Fisheries Science and Technology. 1(2). 1-11.
Hanapin S.HK. 2016. Utilization of Jackfruit (Artocarpus heterophyllus) Leaf Solution with Different Doses Against Bacterial Infections in Dumbo Catfish Larvae (Clarias gariepinus). Thesis. Program, Aquaculture Studies. Faculty of Agriculture. Muhammadiyah University of Makassar. Makassar.
Himmaty, Elfrida Ikvina (2017) Effect of Administration of Rough Extract of Princess Malu Leaf (Mimosa Pudica L.) on Hematology of Koi Fish (Cyprinus Carpio L.) Infected with Pseudomonas Fluorescens Bacteria. Thesis, Brawijaya University.
Igbinosa, O. O., Igbinosa E.O. & O.A. Aiyegoro. 2012. Antimicrobial Activity & phytochemical screening of steam Bark Extracts from (Jatropha curcas linn). African journal of pharmacy and pharmacology.
Ishaqi, A. M. A., and P. D. W. Sari. 2019. Spawning of Koi Fish (Cyprinus Carpio) with Semi Artificial Method: Observation of Fecundity Value, Degree of Egg Fertilization and Egg Hatchability. Journal of Fisheries and Marine 9(2): 216 - 224.
Jannah, N. T., T. W. Agustini, and A. D. Anggo. 2018. Application of putri malu plant extract (Mimosa pudica L.) as an inhibitor of melanosis in shrimp during cold storage. JPB Marine and Fisheries. 13(2): 131-140.
Jun, J.W.; Kim, J.H.; Shin, S.P.; Han, J.E.; Chai, J.Y.; Park, S.C. 2013. Protective effects of the Aeromonas phages pAh1-C and pAh6-C against mass mortality of the cyprinid loach (Misgurnus anguillicaudatus) caused by Aeromonas hydrophila. Aquaculture 2013, 416, 289-295
Kordi, K.M.G.H. 2010. A complete guide to raising freshwater fish in tarpaulin ponds. Agromedia Library. Jakarta.
Kusrini, E., S. Cinderalas, and A. B. Prasetio. 2015. Development of Local Koi (Cyprinus carpio) Ornamental Fish Cultivation at the Depok Ornamental Fish Cultivation Research and Development Center. Aquaculture Media 10(2); 2015.
Lagler, K. F., J. E. Bardach, R. R. Miller and D. R. M. Passino. 1977. Ichthyology. John Wiley and Sons Inc. New York. 506p. Moyle, P.B and Cech Jr, J.J 1988. Fishes. An Introduction to Ichthyology. Prentice Hall, Inc. USA. 559p.
Lakhsmibai R, Amirtham D, and Radhika S. (2015). Preliminary Phytochemical Analysis and Antioxidant Activities of Prosopis Juliflora and Mimosa Pudica Leaves. International Journal of scientific Engineering and Technology Research Vol.04, Issue.30: 5766-5770.
Mali, V. E. R., Y. Jasmanindar, P. Santoso. 2023. Effectiveness of Jackfruit Leaves (Artocarpus heterophyllus) for the Treatment of Goldfish (Cyprinus carpio) Infected with Aeromonas hydrophila Bacteria. Journal of Fisheries, 13 (1), 289-298.
Manik, V. T, Hidayat T, Kusumawaty D. 2014. Identification and phylogenetics of Aeromonas spp bacteria isolated from pond water in several cities based on 16S rRNA gene sequences. Biology Study Program, Department of Biology Education FPMIPA UPI. Bandung.
Masitoh, A. z. 2020. The effectiveness of Sembung leaf extract on the survival of dumbo catfish (clarias gariepinus) infected with aeromonas hydrophila bacteria Journal of Indonesian Swamp Aquaculture. Vol 2 (1): 07-09.
Murwantoko, Rozi, Istiqomah, I., & Nitimulyo, K. H. (2013). Isolation, characterization and pathogenicity of bacteria causing disease in gourami (Osphronemus gouramy) in Bantul Regency. Gadjah Mada University Fisheries Journal, 15(2):83-90.
Napitupulu RA, Suryanto D, Desrita D. 2016. Isolation and Identification of Potential Pathogenic Bacteria in Tilapia (Oreochromis niloticus) in Patumbak Aquaculture Pond. Aquacoastmarine Journal. Vol 15, No 1
Noor, H.F. and Jati, C.W. (2023). EFFECTIVENESS OF INACTIVE VACCINE OF Aeromonas salmonicida AGAINST MASK FISH Cyprinus carpio. Bahari padadak Scientific Journal, 4(1):32-27.
Pal, P., Datta, S., Basnett, H., Shrestha, B., Mohanty, J. P. (2015). Phytochemical analysis of the whole plant of Mimosa pudica (Linn.). UJPSR., 1(1), 1-9.
Parnanto, R. H.R & Rohula, U., Susanto, A. 2013 Effect of Antioxidant & Antibacterial ability of Putri malu Leaf extract on the Quality of Tongkol Fish Fillet. Journal of Food Technoscience Vol 2 No 4.
Pratama RC, Rosidah, Sriati, Ike R. 2017. Effectiveness of rambutan seed extract for treating carp fingerling infected by Aeromonas hydrophila bacteria. Journal Fisheries and Marine 8: 130-136.
Riauwaty, M. and H. Syawal, 2016. Blood Picture of Tilapia (Oreochromis Niloticus) in Aquaculture Ponds in Marpoyan Damai District, Pekanbaru City. Journal of Fisheries and Marine, 21(1); 1-6.
Sanyal, K.B., D. Mukherjee, A. Guchhait, G. Dash. 2018. Phenotypic and molecular identification of bacterial species in Indian major carps and exotic carps from south 24 Parganas, West Bengal, India, Int. J. Curr. Microbiol. Appl. Sci 7 (1) (2018) 534-547,
Saputra, Andis. (2015). Test of Anti-inflammatory Activity of 96% Ethanol Extract of Java Wood Bark (Lannea coromandelica) with Red Blood Cell Membrane Stabilization Method In Vitro. Thesis. Faculty of Medicine and Health Sciences, Syarif State Islamic University.
Styani. H. D., S. Slamet, Wirasti. 2021. Anti-inflammatory Activity of Methanol, Ethyl Acetate, n-Hexan Partition of Putri Malu (Mimosa pudica linn) Leaves. The 13th University Research Colloquium 2021 Sekolah Tinggi Ilmu Kesehatan Muhammadiyah Klaten. Page 916-923.
Subasinghe, R. (2001). Aquaculture development, health and wealth. in aquaculture in the third millennium. Technical Proceedings of The Conference on Aquaculture in the Third Millennium pp. 167-191.
Utami, Mei et al., 2013. Diversity & Utilization of leaves of putri malu (Mimosa pudica). Purwokerto: Faculty of Biology, General Soedirman University Purwokerto.
Widiastuti, I. M. 2009. Growth and Survival Rate of Goldfish (Cyprinus Carpio) Reared in Controlled Containers with Different Stocking Densities. Central Sulawesi Research and Development Media 2 (2): 126-130.
Winker H, Weyl OLF Booth AJ, Ellender BR (2011). Life history and population dynamics of invasive common carp, Cyprinus carpio, in a large turbid African impoundment. Marine and Freshwater Research, 62: 1270-1280.
DOI: https://doi.org/10.35308/ja.v8i2.10297
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