AKTIVITAS INHIBITOR TIROSINASE PADA EKSTRAK ALGA COKELAT SARGASSUM SP. AGARDH ASAL PESISIR LHOK BUBON, KABUPATEN ACEH BARAT

Muhammad Gazali

Abstract


Seaweeds are marine macro algae that can be found attach to the bottom shallow coastal waters with subsrate as attached media. There are three major groups of seaweeds namely brown algae (Phaeophyta), red (Rhodophyta) and green (Chlorophyta). Sargassum sp is one of brown algae which mostly found in the Lhok Bubon Coastal West of Aceh. Recently, exploration of marine macroalgae as bioactive sources was investigated. Seaweed contains bioactive compound which can serve as a defense from ultraviolet radiation that caused hyperpigmentation effect. The aiming of this study is to analyse the tyrosinase inhibitory activity of Sargassum sp extract from Lhok Bubon Coastal Area, West of Aceh. The results shown that the methanol extract of Sargassum sp possess phytochemical properties such as fenol, alkaloid and triterpenoid. Tyrosinase inhibitory activity of Sargassum sp methanol extract  is the best extract which can be inhibit  monophenolase with  IC50 : 1111.49 µg/ml and IC50 = 1582.31 µg/ml in diphenolase pathway with kojic acid as positive control. Moreover, etyl asetate and n-hexane extract have no activity of tyrosinase inhibitor. Therefore, new finding of tyrosinase inhibitor agent from marine macroalgae Sargassum sp give the fruitfull information for cosmeceutical industry.

Keywords : Lhok Bubon, Brown Algae, Sargassum sp, Tyrosinase Inhibitor


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Abbas A. 2006. Minuman Fungsional Berbahan Dasar The dan Kayu Manis untuk Penderita Diabetes. Prosiding pada Seminar Nasional Iptek Solusi Kemandirian Bangsa, 2 – 3 Agustus 2006. Bogor.

Andayani R, Lisawati Y, Maimunah. 2008. Penentuan Aktivitas Antioksidan,Kadar fenolat total, dan likopen pada buah tomat (Solanum lycopersium L). Jurnal Sains dan teknologi Farmasi. 13 (1) : 1 – 9.

[AOAC] Association of Official Analytical Chemist. 2005. Official Method of Analysis of The Association of Official Analytical Chemist, inc

Balboa EM, Conde E, Moure A, et al (2013) In vitro antioxidant properties of crude extracts and compounds from brown algae. Food Chem 138:1764–1785. doi: 10.1016/j.foodchem.2012.11.026

Batubara, I., Darusman, L.K., Mitsunaga, T., Rahminiwati, M., Djauhari, E., 2010. Potency of Indonesia medicinal plant as tyrosinase inhibitor and antioxidant agent. Journal of Biological Science 10:138-144.

Chew YL, Lim YY, Omar M, Khoo Ks. 2008. Antioxidant axtivity of three edible seaweeds from two areas in South East Asia. Libbensmitted – Wissenschaft und Technology 41 : 1067 – 1072.

Chandini SK, Ganesan P, Bhaskar N. 2008. In vitro antioxidant activities of three selected brown seaweeds of India. Food Chemistry 107 : 707 – 713.

Drastinawati Y. 2005. Ekstraksi senyawa metabolit sekunder dari daun tanaman tutup bumi. Jurnal Sains dan Teknologi, 4 : 16 – 19.

Fitrie, A.A., 2004, Histologi dari melanosit. e-USU Repository Universitas Sumatera Utara 5:1-6

Firdaus M, Astawan M, Muchtadi D, Wresdiyati T, Waspadji S, Karyono SS. 2006. Efisiensi anti radikal florotannin Sargassum polycystum dan Sargassum echinocarpum, yang terlarut dalam berbagai pelarut polar. Jurnal Penelitian Perikanan 9 : 157 – 161.

Ganesan P, Kumar CS, Bhaskar N. 2008. Antioxidant properties of methanol extract and its solvent fraction obtained from selected Indian red seaweeds. Bioresource technology 99 : 2717 – 2723.

Hartanti, L., Setiawan, H.K., 2009, Inhibitory potential of some synthetic cinnamic acid derivatives towards tyrosinase enzyme, Indo Journal Chemistry 9:158-168.

Harborne J. 1987. Metode Fitokimia. Edisi ke-2. Kosasih Padmawinata, Penerjemah. Bandung : ITB-Press.

Koche D, Shirsat R, Imran S, Bhadange DG. 2010. Phytochemical screening of eight traditionally used ethnomedicinal plants from Akola District (MS) India. International Journal of Pharma and Bio Sciences 1(4) : 253 – 256.

Lee J-C, Hou M-F, Huang H-W, et al (2013) Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties. Cancer Cell Int 13:55. doi: 10.1186/1475-2867-13-55

Mappiratu. 2009. Kajian Teknologi pengolahan karaginan dari rumput laut Eucheuma cottonii skala rumah tangga. Media Litbang Sulteng 2(1) : 1 – 6.

Manivannan K, Thiumaran G, Karthikai G, Anantharaman P, Balasubramanian T. 2009. Proximate composition of different groupof seaweeds from Vedalai Coastal Waters (Gulf of Mannar) : Southeast Coast of India. Middle – East Journal of Scientific Research 4(2) : 72 – 77.

Miyazawa, M, Tamura, N., 2007, Inhibitory compound of tyrosinase activity from the sprout of Polygonum hydropiper L. (Benitade), Biol Pharm Bull 30:595-597

Montgomery, R., Conway, T.W., Spector, A.A., 1993. Biokimia Beriorientasi pada Kasus Klinik. Staf Pengajar FKUI, penerjemah; Jakarta : Bina rupa Aksara

Muchtadi T, Ayustaningwarno F. 2010. Teknologi Proses Pengolahan Pangan. Bogor ; Alfabeta

Nakai M, Kageyama N, Nakahara K, Miki W. 2006. Phlorotannins as radical scavenger from extract of Sargassum ringgodianum. Marine Biotechnology 8: 409 – 414.

Plaza M, Cifuentes A, Ibanez E (2008) In the search of new functional food ingredients from algae. Trends Food Science Technology 19:31–39.doi: 10.1016/j.tifs.2007.07.012

Shibata T, Kawaguchi S, Hama Y. Inagaki M, Yamaguchi K. Nakamura T. 2004. Local and chemical distribution of phlorotannin in brown algae. Journal of Apply Phycology 16 ; 291 – 296.

Sarastani D. Soekarto S, Muchtadi T, Fardiaz D. Apriyantono A. 2002. Aktivitas Antioksidan Ekstrak dan Fraksi Ekstrak biji atung Parinarium glabberimun Hassk. Jurnal Teknologi dan Industri Pangan 13 (2) : 149 – 156.

Suryanto E, Wehantou F, Raharjo S. 2008. Aktivitas penstabilan senyawa oksigen reaktif dari beberapa herbal. Jurnal Obat Bahan Alam 7(1) : 62 – 68.

Wang T, Jonsdottir R, Olafsdottir G. 2009. Total Phenolic Compound, radical scavanging and metal chelation of extract from Icelandic Seaweed. Food Chemistry 116 : 240 – 248.

Winarno FG. 2008. Kimia Pangan dan Gizi. Bogor : M-Brio Press




DOI: https://doi.org/10.35308/.v1i1.413

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